1. Explain the toxic effect of O2 on strict anaerobes.
With the absence of synthesis catalase, peroxidase, or superoxide dimutase, the toxic concentration of H2O2 cannot be degraded when these organisms are cultivated in the absence of O2.
2. Illustrate the chemical reaction involved in the degradation of hydrogen peroxide in the presence of catalase.
2H2O2 ------> catalase ------> 2H2O + O2
Wednesday, April 2, 2014
Page 216- Questions 3 and 4 Lupe
3. Would
catalase be classified as an endoenzyme or an exoenzyme? Explain
it would be an endoenzyme as it does
not put out into the environment, it has to have H2O2 added to it to work.
4. Account for the ability if
streptococci to tolerate O2 in the absence of catalase activity.
It means that they are facilitative anaerobes that can
survive in both environments, aerobic and anaerobic.
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